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p815 leukemia cells  (ATCC)


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    Structured Review

    ATCC p815 leukemia cells
    ( A ) B6 and BALB/c mice were transplanted with WT or SDHA-KO T cells. Survival rate and clinical GVHD score are shown (syngeneic n = 8/group, allogeneic n = 10/group). ( B – D ) BALB/c mice received HCT with <t>P815</t> cells (MHC class I + class II – ). ( B ) BALB/c mice received syngeneic or allogeneic T cells from WT or SDHA-KO mice and were infused concurrently with 1.0 × 10 3 luciferase + P815 cells. ( C ) Tumor-related mortality (syngeneic n = 5, allogeneic n = 11/group) and clinical GVHD score ( n = 5/group). ( D ) Representative bioluminescence images and tumor burden on day 14 after HCT ( n = 3–10/group). ( E ) BALB/c mice received HCT (WT or SDHA-KO B6 → BALB/c). Representative flow cytometry images and Annexin V + 7-AAD + cells in donor-derived (H-2Kb + ) CD4 + and CD8 + T cells day 7 after HCT ( n = 6/group). ( F ) Representative flow cytometry measuring proliferation of donor-derived (H-2Kb+CD45.2 + ) CD4 + and CD8 + T cells day 7 after HCT ( n = 3–6/group). ( G ) Representative flow cytometry images and granzyme A and perforin levels in donor-derived (H-2Kb + CD45.2 + ) CD8 + T cells day 7 after HCT ( n = 3–10/group). ( H ) Representative flow cytometry images and granzyme A, granzyme B, and perforin levels in donor-derived (H-2Kb + CD45.2 + ) CD4 + T cells day 7 after HCT ( n = 3–10/group). ( I ) WT and SDHA-KO mice were inoculated with B16-F10 melanoma cells on day 0 and injected with anti–PD-1 or isotype control antibody on days 7, 10, 13, and 16. Tumor growth was measured ( n = 6–9/group). Two-tailed Mann-Whitney U test for GVHD score and Annexin V + 7-AAD + , Mantel-Cox log rank test for survival, 1-way ANOVA with Tukey’s post hoc test ( D and F – H ), and 2-way ANOVA with Tukey’s post hoc test ( I ) were used (mean ± SEM). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    P815 Leukemia Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1039 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p815+leukemia+cells/P815/pmc12700539-232-0-8
    Average 96 stars, based on 1039 article reviews
    p815 leukemia cells - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Mitochondrial complex II orchestrates divergent effects in CD4 + and CD8 + T cells"

    Article Title: Mitochondrial complex II orchestrates divergent effects in CD4 + and CD8 + T cells

    Journal: The Journal of Clinical Investigation

    doi: 10.1172/JCI194134

    ( A ) B6 and BALB/c mice were transplanted with WT or SDHA-KO T cells. Survival rate and clinical GVHD score are shown (syngeneic n = 8/group, allogeneic n = 10/group). ( B – D ) BALB/c mice received HCT with P815 cells (MHC class I + class II – ). ( B ) BALB/c mice received syngeneic or allogeneic T cells from WT or SDHA-KO mice and were infused concurrently with 1.0 × 10 3 luciferase + P815 cells. ( C ) Tumor-related mortality (syngeneic n = 5, allogeneic n = 11/group) and clinical GVHD score ( n = 5/group). ( D ) Representative bioluminescence images and tumor burden on day 14 after HCT ( n = 3–10/group). ( E ) BALB/c mice received HCT (WT or SDHA-KO B6 → BALB/c). Representative flow cytometry images and Annexin V + 7-AAD + cells in donor-derived (H-2Kb + ) CD4 + and CD8 + T cells day 7 after HCT ( n = 6/group). ( F ) Representative flow cytometry measuring proliferation of donor-derived (H-2Kb+CD45.2 + ) CD4 + and CD8 + T cells day 7 after HCT ( n = 3–6/group). ( G ) Representative flow cytometry images and granzyme A and perforin levels in donor-derived (H-2Kb + CD45.2 + ) CD8 + T cells day 7 after HCT ( n = 3–10/group). ( H ) Representative flow cytometry images and granzyme A, granzyme B, and perforin levels in donor-derived (H-2Kb + CD45.2 + ) CD4 + T cells day 7 after HCT ( n = 3–10/group). ( I ) WT and SDHA-KO mice were inoculated with B16-F10 melanoma cells on day 0 and injected with anti–PD-1 or isotype control antibody on days 7, 10, 13, and 16. Tumor growth was measured ( n = 6–9/group). Two-tailed Mann-Whitney U test for GVHD score and Annexin V + 7-AAD + , Mantel-Cox log rank test for survival, 1-way ANOVA with Tukey’s post hoc test ( D and F – H ), and 2-way ANOVA with Tukey’s post hoc test ( I ) were used (mean ± SEM). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Figure Legend Snippet: ( A ) B6 and BALB/c mice were transplanted with WT or SDHA-KO T cells. Survival rate and clinical GVHD score are shown (syngeneic n = 8/group, allogeneic n = 10/group). ( B – D ) BALB/c mice received HCT with P815 cells (MHC class I + class II – ). ( B ) BALB/c mice received syngeneic or allogeneic T cells from WT or SDHA-KO mice and were infused concurrently with 1.0 × 10 3 luciferase + P815 cells. ( C ) Tumor-related mortality (syngeneic n = 5, allogeneic n = 11/group) and clinical GVHD score ( n = 5/group). ( D ) Representative bioluminescence images and tumor burden on day 14 after HCT ( n = 3–10/group). ( E ) BALB/c mice received HCT (WT or SDHA-KO B6 → BALB/c). Representative flow cytometry images and Annexin V + 7-AAD + cells in donor-derived (H-2Kb + ) CD4 + and CD8 + T cells day 7 after HCT ( n = 6/group). ( F ) Representative flow cytometry measuring proliferation of donor-derived (H-2Kb+CD45.2 + ) CD4 + and CD8 + T cells day 7 after HCT ( n = 3–6/group). ( G ) Representative flow cytometry images and granzyme A and perforin levels in donor-derived (H-2Kb + CD45.2 + ) CD8 + T cells day 7 after HCT ( n = 3–10/group). ( H ) Representative flow cytometry images and granzyme A, granzyme B, and perforin levels in donor-derived (H-2Kb + CD45.2 + ) CD4 + T cells day 7 after HCT ( n = 3–10/group). ( I ) WT and SDHA-KO mice were inoculated with B16-F10 melanoma cells on day 0 and injected with anti–PD-1 or isotype control antibody on days 7, 10, 13, and 16. Tumor growth was measured ( n = 6–9/group). Two-tailed Mann-Whitney U test for GVHD score and Annexin V + 7-AAD + , Mantel-Cox log rank test for survival, 1-way ANOVA with Tukey’s post hoc test ( D and F – H ), and 2-way ANOVA with Tukey’s post hoc test ( I ) were used (mean ± SEM). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Techniques Used: Luciferase, Flow Cytometry, Derivative Assay, Injection, Control, Two Tailed Test, MANN-WHITNEY

    Related Articles

    Transduction:

    Article Title: A novel role for the semaphorin Sema4D in the induction of alloresponses
    Article Snippet: Two or three-color flow cytometric analysis was performed by using FACS Vantage or FACS Diva SE cell sorter (Becton Dickinson, San Jose, CA). .. P815 leukemia cells were purchased from ATCC (Virginia) and were transduced with a third generation lentivirus co-expressing GFP and firefly luciferase (Luc) ( 23 , 24 ). ..

    Article Title: Mitochondrial complex II orchestrates divergent effects in CD4 + and CD8 + T cells
    Article Snippet: .. P815 leukemia cells (catalog TIB-64) were purchased from ATCC and were transduced with luciferase ( ). ..

    Luciferase:

    Article Title: A novel role for the semaphorin Sema4D in the induction of alloresponses
    Article Snippet: Two or three-color flow cytometric analysis was performed by using FACS Vantage or FACS Diva SE cell sorter (Becton Dickinson, San Jose, CA). .. P815 leukemia cells were purchased from ATCC (Virginia) and were transduced with a third generation lentivirus co-expressing GFP and firefly luciferase (Luc) ( 23 , 24 ). ..

    Article Title: Mitochondrial complex II orchestrates divergent effects in CD4 + and CD8 + T cells
    Article Snippet: .. P815 leukemia cells (catalog TIB-64) were purchased from ATCC and were transduced with luciferase ( ). ..



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    ( A ) B6 and BALB/c mice were transplanted with WT or SDHA-KO T cells. Survival rate and clinical GVHD score are shown (syngeneic n = 8/group, allogeneic n = 10/group). ( B – D ) BALB/c mice received HCT with <t>P815</t> cells (MHC class I + class II – ). ( B ) BALB/c mice received syngeneic or allogeneic T cells from WT or SDHA-KO mice and were infused concurrently with 1.0 × 10 3 luciferase + P815 cells. ( C ) Tumor-related mortality (syngeneic n = 5, allogeneic n = 11/group) and clinical GVHD score ( n = 5/group). ( D ) Representative bioluminescence images and tumor burden on day 14 after HCT ( n = 3–10/group). ( E ) BALB/c mice received HCT (WT or SDHA-KO B6 → BALB/c). Representative flow cytometry images and Annexin V + 7-AAD + cells in donor-derived (H-2Kb + ) CD4 + and CD8 + T cells day 7 after HCT ( n = 6/group). ( F ) Representative flow cytometry measuring proliferation of donor-derived (H-2Kb+CD45.2 + ) CD4 + and CD8 + T cells day 7 after HCT ( n = 3–6/group). ( G ) Representative flow cytometry images and granzyme A and perforin levels in donor-derived (H-2Kb + CD45.2 + ) CD8 + T cells day 7 after HCT ( n = 3–10/group). ( H ) Representative flow cytometry images and granzyme A, granzyme B, and perforin levels in donor-derived (H-2Kb + CD45.2 + ) CD4 + T cells day 7 after HCT ( n = 3–10/group). ( I ) WT and SDHA-KO mice were inoculated with B16-F10 melanoma cells on day 0 and injected with anti–PD-1 or isotype control antibody on days 7, 10, 13, and 16. Tumor growth was measured ( n = 6–9/group). Two-tailed Mann-Whitney U test for GVHD score and Annexin V + 7-AAD + , Mantel-Cox log rank test for survival, 1-way ANOVA with Tukey’s post hoc test ( D and F – H ), and 2-way ANOVA with Tukey’s post hoc test ( I ) were used (mean ± SEM). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    P815 Leukemia Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p815+leukemia+cells/P815/pmc12700539-232-0-8
    Average 96 stars, based on 1 article reviews
    p815 leukemia cells - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    luciferase p815 cell line p815 leukemia cells  (ATCC)
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    ATCC luciferase p815 cell line p815 leukemia cells
    ( A ) B6 and BALB/c mice were transplanted with WT or SDHA-KO T cells. Survival rate and clinical GVHD score are shown (syngeneic n = 8/group, allogeneic n = 10/group). ( B – D ) BALB/c mice received HCT with <t>P815</t> cells (MHC class I + class II – ). ( B ) BALB/c mice received syngeneic or allogeneic T cells from WT or SDHA-KO mice and were infused concurrently with 1.0 × 10 3 luciferase + P815 cells. ( C ) Tumor-related mortality (syngeneic n = 5, allogeneic n = 11/group) and clinical GVHD score ( n = 5/group). ( D ) Representative bioluminescence images and tumor burden on day 14 after HCT ( n = 3–10/group). ( E ) BALB/c mice received HCT (WT or SDHA-KO B6 → BALB/c). Representative flow cytometry images and Annexin V + 7-AAD + cells in donor-derived (H-2Kb + ) CD4 + and CD8 + T cells day 7 after HCT ( n = 6/group). ( F ) Representative flow cytometry measuring proliferation of donor-derived (H-2Kb+CD45.2 + ) CD4 + and CD8 + T cells day 7 after HCT ( n = 3–6/group). ( G ) Representative flow cytometry images and granzyme A and perforin levels in donor-derived (H-2Kb + CD45.2 + ) CD8 + T cells day 7 after HCT ( n = 3–10/group). ( H ) Representative flow cytometry images and granzyme A, granzyme B, and perforin levels in donor-derived (H-2Kb + CD45.2 + ) CD4 + T cells day 7 after HCT ( n = 3–10/group). ( I ) WT and SDHA-KO mice were inoculated with B16-F10 melanoma cells on day 0 and injected with anti–PD-1 or isotype control antibody on days 7, 10, 13, and 16. Tumor growth was measured ( n = 6–9/group). Two-tailed Mann-Whitney U test for GVHD score and Annexin V + 7-AAD + , Mantel-Cox log rank test for survival, 1-way ANOVA with Tukey’s post hoc test ( D and F – H ), and 2-way ANOVA with Tukey’s post hoc test ( I ) were used (mean ± SEM). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Luciferase P815 Cell Line P815 Leukemia Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p815+leukemia+cells/P815/pmc02278022-86-0-10
    Average 96 stars, based on 1 article reviews
    luciferase p815 cell line p815 leukemia cells - by Bioz Stars, 2026-10
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    Image Search Results


    ( A ) B6 and BALB/c mice were transplanted with WT or SDHA-KO T cells. Survival rate and clinical GVHD score are shown (syngeneic n = 8/group, allogeneic n = 10/group). ( B – D ) BALB/c mice received HCT with P815 cells (MHC class I + class II – ). ( B ) BALB/c mice received syngeneic or allogeneic T cells from WT or SDHA-KO mice and were infused concurrently with 1.0 × 10 3 luciferase + P815 cells. ( C ) Tumor-related mortality (syngeneic n = 5, allogeneic n = 11/group) and clinical GVHD score ( n = 5/group). ( D ) Representative bioluminescence images and tumor burden on day 14 after HCT ( n = 3–10/group). ( E ) BALB/c mice received HCT (WT or SDHA-KO B6 → BALB/c). Representative flow cytometry images and Annexin V + 7-AAD + cells in donor-derived (H-2Kb + ) CD4 + and CD8 + T cells day 7 after HCT ( n = 6/group). ( F ) Representative flow cytometry measuring proliferation of donor-derived (H-2Kb+CD45.2 + ) CD4 + and CD8 + T cells day 7 after HCT ( n = 3–6/group). ( G ) Representative flow cytometry images and granzyme A and perforin levels in donor-derived (H-2Kb + CD45.2 + ) CD8 + T cells day 7 after HCT ( n = 3–10/group). ( H ) Representative flow cytometry images and granzyme A, granzyme B, and perforin levels in donor-derived (H-2Kb + CD45.2 + ) CD4 + T cells day 7 after HCT ( n = 3–10/group). ( I ) WT and SDHA-KO mice were inoculated with B16-F10 melanoma cells on day 0 and injected with anti–PD-1 or isotype control antibody on days 7, 10, 13, and 16. Tumor growth was measured ( n = 6–9/group). Two-tailed Mann-Whitney U test for GVHD score and Annexin V + 7-AAD + , Mantel-Cox log rank test for survival, 1-way ANOVA with Tukey’s post hoc test ( D and F – H ), and 2-way ANOVA with Tukey’s post hoc test ( I ) were used (mean ± SEM). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: The Journal of Clinical Investigation

    Article Title: Mitochondrial complex II orchestrates divergent effects in CD4 + and CD8 + T cells

    doi: 10.1172/JCI194134

    Figure Lengend Snippet: ( A ) B6 and BALB/c mice were transplanted with WT or SDHA-KO T cells. Survival rate and clinical GVHD score are shown (syngeneic n = 8/group, allogeneic n = 10/group). ( B – D ) BALB/c mice received HCT with P815 cells (MHC class I + class II – ). ( B ) BALB/c mice received syngeneic or allogeneic T cells from WT or SDHA-KO mice and were infused concurrently with 1.0 × 10 3 luciferase + P815 cells. ( C ) Tumor-related mortality (syngeneic n = 5, allogeneic n = 11/group) and clinical GVHD score ( n = 5/group). ( D ) Representative bioluminescence images and tumor burden on day 14 after HCT ( n = 3–10/group). ( E ) BALB/c mice received HCT (WT or SDHA-KO B6 → BALB/c). Representative flow cytometry images and Annexin V + 7-AAD + cells in donor-derived (H-2Kb + ) CD4 + and CD8 + T cells day 7 after HCT ( n = 6/group). ( F ) Representative flow cytometry measuring proliferation of donor-derived (H-2Kb+CD45.2 + ) CD4 + and CD8 + T cells day 7 after HCT ( n = 3–6/group). ( G ) Representative flow cytometry images and granzyme A and perforin levels in donor-derived (H-2Kb + CD45.2 + ) CD8 + T cells day 7 after HCT ( n = 3–10/group). ( H ) Representative flow cytometry images and granzyme A, granzyme B, and perforin levels in donor-derived (H-2Kb + CD45.2 + ) CD4 + T cells day 7 after HCT ( n = 3–10/group). ( I ) WT and SDHA-KO mice were inoculated with B16-F10 melanoma cells on day 0 and injected with anti–PD-1 or isotype control antibody on days 7, 10, 13, and 16. Tumor growth was measured ( n = 6–9/group). Two-tailed Mann-Whitney U test for GVHD score and Annexin V + 7-AAD + , Mantel-Cox log rank test for survival, 1-way ANOVA with Tukey’s post hoc test ( D and F – H ), and 2-way ANOVA with Tukey’s post hoc test ( I ) were used (mean ± SEM). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: P815 leukemia cells (catalog TIB-64) were purchased from ATCC and were transduced with luciferase ( ).

    Techniques: Luciferase, Flow Cytometry, Derivative Assay, Injection, Control, Two Tailed Test, MANN-WHITNEY